Effects of peptides of pituitary origin on the formation of C21 steroid by fetal calf adrenal cells in culture. |
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| Authors: | K W Kan A Baird N G Seidah M Lis M Chrétien N Ling R Guillemin S Solomon |
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| Institution: | 1. Departments of Biochemistry and Experimental Medicine, McGill University and Royal Victoria Hospital and the Protein Pituitary Hormone Laboratory, Clinical Research Institute of Montreal, Canada;2. Salk Institute, La Jolla, California, USA |
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| Abstract: | Corticotrophic activity of opiate-like peptides was assessed by their ability to stimulate the formation of C21 steroids from 3H] progesterone by three-day old cultures of fetal calf adrenal cells. ACTH1–39, ACTHα1–24 and a purified preparation of pituitary ovine β-endorphin caused a marked increase in 17α and 21-hydroxylation while a preparation of pure synthetic porcine β-endorphin gave a minimal stimulation. The activity of the purified ovine β-endorphin preparation could not be accounted for by contamination by ACTH or by a synergistic action between the two peptides. The novel pituitary factor described here may be due to a contaminant of the β-endorphin peak which is different from ACTH1–39. |
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| Keywords: | β-LPH β-lipotròphic hormone F cortisol B corticosterone S 11-deoxycortisol DOC 11-deoxycorticosterone 17-OH-P 17α-hydroxyprogesterone P progesterone TLC thin layer chromatography |
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