Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli |
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| Authors: | S Aoyama N Yoshida S Inouye |
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| Affiliation: | Research Center, Chisso Petrochemical Corporation, Chiba, Japan. |
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| Abstract: | The lipase gene from Pseudomonas fragi IFO-12049 was isolated using the expression library and the primary structure of lipase deduced from the nucleotide sequence was determined. It is composed of 277 amino acid residues and a protein of Mr 29,966, which was close to the value of the lipase expressed in E. coli. |
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