Optimization of phycocyanin extraction from Spirulina platensis using factorial design |
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| Authors: | Silveira S T Burkert J F M Costa J A V Burkert C A V Kalil S J |
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| Institution: | 1. Departmento de Biología, Facultad de Química, UNAM, Ciudad Universitaria, México, D.F., 04510, México;2. Unidad de Investigación Médica en Trombosis, Hemostasia y Aterogénesis del Hospital General Regional, No. 1 Dr. Dr. Carlos Mac Gregor Sánchez Navarro, IMSS, México, D.F., México;1. Laboratory of Plants Production Biotechnology, Biotechnology Department, Nature and Life Sciences Faculty, Blida 1 University, Algeria;2. Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. Box 1173, Sfax 3038, Tunisia;3. European Institute of Membranes, UMR 5635, Montpellier 2 University, France;4. Institute of Technology of Agricultural Products, Hellenic Agricultural Organization – Demeter (ELGO-Demeter), Leof, SofokliVenizelou 1, Lykovrysi 141 23, Greece |
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| Abstract: | Phycocyanin extraction from cyanobacteria Spirulina platensis was optimized using factorial design and response surface techniques. The effects of temperature and biomass-solvent ratio on phycocyanin concentration and extract purity were evaluated to determine the optimum conditions for phycocyanin extraction. The optimum conditions for the extraction of phycocyanin from S. platensis were the highest biomass-solvent ratio, 0.08 gmL(-1), and 25 degrees C. Under these conditions it's possible to obtain an extract of phycocyanin with a concentration of 3.68 mgmL(-1) and purity ratio (A(615)/A(280)) of 0.46. |
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