Clonal Diversity in Introduced Populations of An Asian Sea Anemone in North America |
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Authors: | Joy H. Ting Jonathan B. Geller |
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Affiliation: | (1) Instituto Argentino de Oceanograf?a, Consejo Nacional de Investigaciones Cient?ficas y T?cnicas (CONICET), CC 804, B8000FWB, Bahia Blanca, Argentina;(2) Consejo Nacional de Investigaciones Cient?ficas y T?cnicas (CONICET), Buenos Aires, Argentina;(3) Laboratorio de Ecolog?a, Depto. de Biolog?a (FCEyN), Universidad Nacional de Mar del Plata, CC 573 Correo Central, B7600WAG, Mar del Plata, Argentina;(4) Departamento de Biolog?a, Bioqu?mica y Farmacia, Universidad Nacional del Sur, San Juan 670, 8000 Bahia Blanca, Argentina;(5) Departamento de Ciencias Marinas, Universidad Nacional de Mar del Plata, De?n Funes 3350, B 76002 AYL, Mar del Plata, Argentina;(6) Departamento de Geolog?a, Universidad Nacional del Sur, San Juan 670, 8000 Bahia Blanca, Argentina |
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Abstract: | Previous reports hypothesized that introduced populations of the Asian sea anemone Diadumene lineata (Verill, 1870 (Presented 1869) Communications of the Essex Institution 6: 51–104), which reproduces by fission, are often monoclonal or to be composed of few clones. To test this hypothesis, sea anemones were collected from thirteen sites in three non-native regions and one native region: Chesapeake Bay, New England, central California, and Japan. The internal transcribed spacer (ITS) region separating nuclear ribosomal RNA genes was amplified from each individual using PCR and surveyed for DNA sequence variation using single strand conformational polymorphism analysis (SSCP). Fifty-six distinct electrophoretic banding patterns were found in 268 anemones, and each pattern was considered a different genotype. The number of genotypes in a population ranged from one to thirteen. Only one sample (York River, Chesapeake Bay, n = 10) was monoclonal, although six populations were dominated (>50%) by single genotype. Only four genotypes were found in more than one population, and these were confined to single regions. Walker Creek, California was sampled in 1995 and 1997 and no genotypes were found in both years, suggesting rapid shifts in genotype frequency. We conclude that multiple genotypes of D. lineata have invaded North America and that the primary importance of clonal growth for introduced populations is the production of colonizing propagules. |
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Keywords: | Diadumene lineata genotypic diversity Haliplanella lineata ITS ribosomal RNA SSCP |
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