A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars |
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Authors: | A Prevost M J Wilkinson |
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Institution: | (1) Department of Agricultural Botany, School of Plant Sciences, The University of Reading, Whiteknights, P.O. Box 221, Reading, RG6 6AS, UK, GB |
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Abstract: | Commercial scale fingerprinting of potato cultivars is made difficult by the need for speed, reliability and the ability
to distinguish between large numbers of genotypes. There are also problems in extrapolating the results of small experimental
studies to predict the performance of techniques or primers for larger applications. The potential of ISSR-PCR for fingerprinting
purposes was evaluated using four primers on 34 potato cultivars. The complex band profiles generated were reproducible between
repeat PCRs, DNA extractions, electrophoreses and gel scorings. Two primers were each able to distinguish all cultivars. The
combined use of any two of the four primers also allowed complete diagnosis. It is concluded that ISSR-PCR provides a quick,
reliable and highly informative system for DNA fingerprinting that is amenable for routine applications. Two possible correlates
of the ability of primers to distinguish between genotypes were then examined. Marker Index failed to correlate significantly
with genotype diagnosis, but a strong and seemingly linear relationship was observed between Resolving Power of a primer and
its ability to distinguish genotypes (r2=0.98). Resolving Power of one or a pair of primers was found to provide a moderately accurate estimate of the number of genotypes
identified. Possible implications for future studies on DNA fingerprinting are discussed.
Received: 7 May 1998 / Accepted: 15 July 1998 |
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Keywords: | DNA fingerprinting ISSR-PCR Solanum tuberosum Resolving Power |
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