A scanning near-field optical microscope approach to biomolecule patterning |
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Authors: | Philipona C Chevolot Y Léonard D Mathieu H J Sigrist H Marquis-Weible F |
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Institution: | Institut d'Optique Appliquée, Ecole polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. |
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Abstract: | In view of future generations of biosensors and advanced biomaterials, photochemistry in the near field using scanning near-field optical microscopy is investigated. The potential of direct near-field-induced photoactivation is demonstrated on standard photoresist. Photoimmobilization of maleimidoaryldiazirine on silicon substrates and bovine serum albumin on glass substrates is achieved, opening the way to a controlled biopatterning of surfaces with submicrometer feature size. The obtained patterns are characterized using atomic force microscopy, time-of-flight secondary ion mass spectroscopy (ToF-SIMS), and near-field fluorescence microscopy. |
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