Detection by DGGE of a new polymorphism closely linked to the adenomatous polyposis coli region |
| |
Authors: | Sylviane Olschwang Richard Fabre Pierre Laurent-Puig Anne Vassal Bernard Hamelin Yusuke Nakamura Gilles Thomas |
| |
Affiliation: | (1) Laboratoire de Génétique des Tumeurs, Institut Curie, 26, Rue d'Ulm, F-75231 Paris Cedex 05, France;(2) Cancer Institute, 170 Tokyo, Japan |
| |
Abstract: | Summary The EF5.44 locus is in close proximity to the chromosome 5 region to which the genetic defect responsible for familial adenomatous polyposis has been mapped. We have devised two oligonucleotides that promote the specific polymerase chain reaction (PCR) amplificiation of a 365-bp sequence in this region. Analysis by denaturing gradient gel electrophoresis of the resulting fragment has unravelled individual differences that could be identified as a single base pair change in aMnlI restriction site. This PCR assayable polymorphism increases the informativeness at this locus, and should be useful in the presymptomatic diagnosis of familial adenomatous polyposis. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|