Definition by CB12 monoclonal antibody of a differentiation marker specific for human monocytes and their bone marrow precursors |
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| Authors: | F Malavasi A Funaro G Bellone F Caligaris-Cappio G Semenzato A P Cappa E Ferrero F Novelli M Alessio S Demaria |
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| Affiliation: | 1. Cattedra di Biologia Cellulare, Istituto di Genetica Medica, and Clinica Medica, Università di Torino, Torino, Italy;2. Istituto di Medicina Clinica, Clinica Medica I∘ e Cattedra di Immunologia Clinica, Università di Padava, Padova, Italy;3. Servizio di Anatomia ed Istologia Patologica, Ospedale San Giovanni, Torino, Italy;4. Centro di Immunogenetica ed Istocompatibilità, CNR, Torino, Italy;1. Clinic of Gastroenterology, Hepatology and Infectious Diseases, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany;2. Host-Pathogen Interactions, Paul-Ehrlich-Institut, Langen, Germany;3. Institute for Pharmaceutical and Medicinal Chemistry, Heinrich Heine University Düsseldorf, Düsseldorf, Germany;4. Center for Structural Studies (CSS), Heinrich Heine University Düsseldorf, Düsseldorf, Germany;5. Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany;6. Department of Molecular Medicine II, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany;7. Institute for Virology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany;8. Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany;9. REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany;10. Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Hannover Medical School, 30625 Hannover, Germany;11. Institute of Bio- and Geosciences (IBG-4: Bioinformatics), Forschungszentrum Jülich GmbH, 52425 Jülich, Germany;1. Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA 94143, USA;2. Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA;3. J. David Gladstone Institutes, San Francisco, CA 94158, USA;4. Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA;5. Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA;6. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA;7. Quantitative Biosciences Institute, QBI, University of California, San Francisco, San Francisco, CA 94158, USA;8. Department of Medicine, Division of Experimental Medicine, University of California, San Francisco, San Francisco, CA 94143, USA;9. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA;10. Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA;11. Vitalant Research Institute, San Francisco, CA 94118, USA;12. Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA;13. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA;14. Division of Infectious Diseases, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA;15. Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA;16. Parker Institute for Cancer Immunotherapy, San Francisco, CA 94129, USA;17. UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA 94158, USA;18. Chan Zuckerberg Biohub, San Francisco, CA 94158, USA |
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| Abstract: | The CB12 monoclonal antibody, which reacts with a molecule expressed on monocytes, was characterized using human embryonic material as immunizer. Analysis of the monoclonal antibody at the phenotypic, molecular, and functional levels indicates that its reactivity is restricted to circulating monocytes and their precursors in the bone marrow, whereas it is undetectable on tissue macrophages. CB12 displays a pattern of reactivity compatible with that of a marker of monocyte differentiation. Preliminary data indicate a possible receptor role for the CB12 molecule. |
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