Molecular analyses of the rice <Emphasis Type="Italic">glutamate dehydrogenase</Emphasis> gene family and their response to nitrogen and phosphorous deprivation |
| |
Authors: | Xuhua Qiu Weibo Xie Xingming Lian Qifa Zhang |
| |
Institution: | (1) National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070 Wuhan, China |
| |
Abstract: | Glutamate dehydrogenases (GDH, EC 1.4.1.2~4) are ubiquitous enzymes encoded by GDH genes. So far, at least two GDH members have been characterized in plants, but most members of this family in rice remains to be characterized. Here, we
show that four putative GDH genes (OsGDH1-4) are present in the rice genome. The GDH sequences from rice and other species can be classified into two types (I and II).
OsGDH1-3 belonged to type II genes, whereas OsGDH4 belonged to type I like gene. Our data implied that the expansion rate of type I genes was much slower than that of type
II genes and species-specific expansion contributed to the evolution of type II genes in plants. The expression levels of
the different members of GDH family in rice were evaluated using quantitative real-time PCR and microarray analysis. Gene expression patterns revealed
that OsGDH1, OsGDH2, and OsGDH4 are expressed ubiquitously in various tissues, whereas OsGDH3 expression is glumes and stamens specific. The expression of the OsGDH family members responded differentially to nitrogen and phosphorus-deprivation, indicating their roles under such stress
conditions. Implications of the expression patterns with respect to the functions of these genes were discussed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Glutamate dehydrogenase (GDH) Nitrogen deprivation Phosphorus deprivation Phylogenetic analysis Rice (Oryza sativa) |
本文献已被 SpringerLink 等数据库收录! |
|