| 3-羰基辛酰基高丝氨酸内酯诱导拟南芥根细胞Ca~（2＋）内流 |
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| 引用本文： | 张哲,张霞,边子睿,宋水山.3-羰基辛酰基高丝氨酸内酯诱导拟南芥根细胞Ca~（2＋）内流[J].植物生理学通讯,2011(9):872-878. |
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| 作者姓名： | 张哲 张霞 边子睿 宋水山 |
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| 作者单位： | 河北省科学院生物研究所;河北省农作物主要病害微生物控制工程技术研究中心;河北工业大学化工学院; |
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| 基金项目： | 国家重点基础研究发展计划(2009CB126010); 河北省自然科学基金(C2006000707); 河北省重点基础研究计划项目(08965506D) |
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| 摘 要： | N-酰基高丝氨酸内酯（AHLs）是革兰氏阴性细菌群体感应系统（QS）中的胞间通讯信号分子。近年的研究表明AHLs可以调控植物生长发育及防卫反应,但其调控机制尚不清楚。本研究以拟南芥为材料,采用3-羰基辛酰基高丝氨酸内酯（3OC8-HSL）处理转水母发光蛋白基因的拟南芥幼根细胞,利用冷光仪检测3OC8-HSL对拟南芥根细胞中胞质游离Ca2＋浓度（Ca2＋]cyt）变化的影响,同时采用Ca2＋专一性螯合剂EGTA和Ca2＋通道抑制剂预处理转基因拟南芥根细胞,用全细胞膜片钳技术分析3OC8-HSL诱导拟南芥根细胞中Ca2＋]cyt升高的Ca2＋来源。结果表明,3OC8-HSL可诱导拟南芥根细胞中Ca2＋]cyt瞬时升高。这种诱导效应可被EGTA、异搏定（verapamil）、LaCl3所抑制,但LiCl预处理对这种诱导效应无影响。膜片钳分析结果显示,3OC8-HSL可激活质膜Ca2＋通道,增加胞外Ca2＋内流。说明细菌AHLs可诱导植物Ca2＋信号产生,且这种Ca2＋信号主要源于胞外Ca2＋内流,暗示Ca2＋信使系统参与植物对细菌QS信号的响应。
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| 关 键 词： | N-酰基高丝氨酸内酯 细菌群体感应 钙信使 拟南芥 |
N-3-oxo-Octanoyl-Homoserine Lactone Induces Intracellular Ca~(2+) Elevation in Arabidopsis Root Cells |
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| ZHANG Zhe,ZHANG Xia,BIAN Zi-Rui,SONG Shui-Shan.N-3-oxo-Octanoyl-Homoserine Lactone Induces Intracellular Ca~(2+) Elevation in Arabidopsis Root Cells[J].Plant Physiology Communications,2011(9):872-878. |
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| Authors: | ZHANG Zhe ZHANG Xia BIAN Zi-Rui SONG Shui-Shan |
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| Institution: | 1,2,？ 1Biology Institute, Hebei Academy of Sciences, Shijiazhuang 050051, China; 2Hebei Engineering and Technology Center of Microbiological Control on Main Crop Disease, Shijiazhuang 050051, China; 3School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China |
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| Abstract: | N-acyl-L-homoserine lactones (AHLs) are the signal molecules which mediate the quorum-sensing (QS) in Gram-negative bacteria. In recent years, the accumulating evidence indicates that AHLs are able to regulate the growth, development and defense response of plants. However, the mechanisms by which plant responses to bacterial QS signal molecule is little known. In this study, the change of cytosolic free Ca2+ concentration (Ca2+]cyt) and the activities of calcium channel in Arabidopsis thaliana root cells ... |
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| Keywords: | N-acyl-homoserine lactones bacterial quorum-sensing Ca2 signaling Arabidopsis thaliana |
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