| 秋水仙素诱导大青杨同源三倍体 |
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| 引用本文： | 娄玲玲,赵慧,祁传磊,卢倩倩,王勃,李开隆. 秋水仙素诱导大青杨同源三倍体[J]. 植物生理学通讯, 2011, 0(7): 699-704 |
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| 作者姓名： | 娄玲玲 赵慧 祁传磊 卢倩倩 王勃 李开隆 |
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| 作者单位： | 东北林业大学林学院林木遗传育种国家重点实验室; |
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| 基金项目： | 黑龙江省“十一五”科技攻关项目(GA06B301-2); 大学生创新课题(101022542) |
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| 摘 要： | 以黑龙江省苇河、带岭的大青杨为亲本，采用正交设计L16（4^5）对授粉后的雌花序施加0．2％～O．6％的秋水仙碱溶液进行染色体加倍试验，通过流式细胞术和染色体镜检方法检测变异植株的染色体倍性，共获得12株大青杨同源三倍体。结果表明，在授粉36～60h后用O．5％秋水仙碱溶液浸泡24h的诱导效果最好。大青杨三倍体植株叶长和叶宽分别高出二倍体2l％和45％；其叶片气孔长、宽高出二倍体55％和45％，气孔密度是二倍体的18％。
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| 关 键 词： | 大青杨 秋水仙素 同源三倍体 |
Autotriploid Induction in Populus ussuriensis Kom.by Colchicine |
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| LOU Ling-Ling,ZHAO Hui,QI Chuan-Lei,LU Qian-Qian,WANG Bo,LI Kai-Long State Key Laboratory of Forest Genetics , Tree Breeding,Northeast Forestry University,Harbin ,China. Autotriploid Induction in Populus ussuriensis Kom.by Colchicine[J]. Plant Physiology Communications, 2011, 0(7): 699-704 |
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| Authors: | LOU Ling-Ling ZHAO Hui QI Chuan-Lei LU Qian-Qian WANG Bo LI Kai-Long State Key Laboratory of Forest Genetics Tree Breeding Northeast Forestry University Harbin China |
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| Affiliation: | LOU Ling-Ling,ZHAO Hui,QI Chuan-Lei,LU Qian-Qian,WANG Bo,LI Kai-Long* State Key Laboratory of Forest Genetics and Tree Breeding,Northeast Forestry University,Harbin 150040,China |
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| Abstract: | With the parents of Populus ussuriensis from Dailing and Weihe forest bureau in Heilongjiang,the female inflorescences were treated with 0.2%-0.6% colchicine after pollinating to induce chromosome doubling by orthogonal design of L16(45),and 12 triploid plants were obtained by flow cytometry and microscopic to detect chromosomal ploidy.The results showed that the most effective induction was 0.5% colchicine for 24 h at 36-60 h after pollination.The length and width of triploid leaves were 21% and 45% respec... |
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| Keywords: | Populus ussuriensis colchicine autotriploid |
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