| pSP6—LUC融合质粒的构建、鉴定和制备 |
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| 引用本文： | 傅明辉,金振华. pSP6—LUC融合质粒的构建、鉴定和制备[J]. 生物学杂志, 2001, 18(4): 21-22,24 |
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| 作者姓名： | 傅明辉 金振华 |
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| 作者单位： | 1. 广东汕头大学生物系,汕头,515063
2. 中科院发育所,北京,100080 |
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| 摘 要： | 以pSP64为载体，插入荧光素酶基因片断构成pSP6-LUC融合质粒，并将其转化E.ColiHB101鉴定后大最制备融合质粒。
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| 关 键 词： | 荧光素酶基因 融合质粒 转化 鉴定 制备 |
| 文章编号： | 1008-9632(2001)04-0021-02 |
The construction,identification and isolation of pSP-LUC fusion plasmid |
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| FU Ming-hui ,JIN Zhen-hua. The construction,identification and isolation of pSP-LUC fusion plasmid[J]. Journal of Biology, 2001, 18(4): 21-22,24 |
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| Authors: | FU Ming-hui JIN Zhen-hua |
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| Affiliation: | FU Ming-hui 1,JIN Zhen-hua 2 |
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| Abstract: | Luciferase gene was inserted into the vecror,pSP64 to construct the fusion plasmid,pSP6-LUC,and then the fusion plasmid was transformed into E.coliHB101,after identification,we could large scale isolated this fusion plasmid. |
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| Keywords: | luciferase gene the fusion plasmid transform. |
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