| 球孢白僵菌热休克蛋白基因Bbhsp70的cDNA及上游序列克隆与分析 |
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| 引用本文： | 谢 翎 陈红梅 蒲顺昌 汤 强 李增智 黄 勃. 球孢白僵菌热休克蛋白基因Bbhsp70的cDNA及上游序列克隆与分析[J]. 菌物学报, 2009, 28(2): 283-288 |
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| 作者姓名： | 谢 翎 陈红梅 蒲顺昌 汤 强 李增智 黄 勃 |
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| 作者单位： | 安徽农业大学微生物防治省重点实验室,合肥,230036;安庆师范学院生命科学系,安庆,246011 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，教育部新世纪优秀人才支持计划，安徽省优秀青年科技基金，安徽省级自然科学研究重点项目 |
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| 摘 要： | 运用SMART RACE RT-PCR技术与DNA步移技术,首次从球孢白僵菌中克隆出完整的热休克蛋白基因Bbhsp70编码区序列及上游序列。该基因cDNA全长2405bp,5′端非翻译区171bp,3′端非翻译区263bp,开放阅读框(ORF)1971bp,编码656个氨基酸。成熟蛋白理论分子量为71.3kDa,理论等电点为4.92。上游序列长度3559bp,其中有305bp序列与cDNA序列重叠。分析表明,上游序列中没有明显的TATA-盒和CAAT-盒,但含有CCAAT-bindingfactor、GC-box等重要的转录因子结合位点,以及热激应答元件(HSE)和GATA元件等启动子顺式调控元件。
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| 关 键 词： | 昆虫病原真菌 RACE DNA步移 上游调控序列 |
Cloning and sequence analysis of cDNA and upstream sequence of Bbhsp70 gene from Beauveria bassiana |
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| XIE Ling CHEN Hong-Mei PU Shun-Chang TANG Qiang LI Zeng-Zhi HUANG Bo. Cloning and sequence analysis of cDNA and upstream sequence of Bbhsp70 gene from Beauveria bassiana[J]. Mycosystema, 2009, 28(2): 283-288 |
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| Authors: | XIE Ling CHEN Hong-Mei PU Shun-Chang TANG Qiang LI Zeng-Zhi HUANG Bo |
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| Affiliation: | Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | The full-length cDNA of Bbhsp70 gene was cloned from Beauveria bassiana using SMART RACE RT-PCR, and its upstream sequence was amplified using genome walking. Analysis of the cloned complete cDNA with a whole length of 2405bp showed that it encompassed a open reading frame (ORF) with 1971bp encoding 656 amino acid. The mature protein had a molecular mass of 71.3kDa with a calculated pI of 4.92. The analyses indicate that the upstream sequence contained several regulatory elements, such as CCAAT-binding fact... |
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| Keywords: | RACE |
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