Coexpression of embryonic, fetal, and adult globins in erythroid cells of human embryos: relevance to the cell-lineage models of globin switching |
| |
| Authors: | G Stamatoyannopoulos P Constantoulakis M Brice S Kurachi T Papayannopoulou |
| |
| Affiliation: | 1. Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195 USA;1. Division of Hematology, Department of Medicine, University of Washington, Seattle, Washington 98195 USA;1. Department of Anesthesiology, Perioperative, and Pain Medicine, Stanford University School of Medicine, Stanford, CA, USA;2. Department of Anaesthesia and Pain Medicine, King Edward Memorial Hospital, Perth, WA, Australia;1. Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran;2. Department of Biochemistry and Biophysics, Metabolic Disorders Research Center, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan Province, Iran;3. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain;5. Zabol University of Medical Sciences, Zabol, Iran;6. Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran;7. Department of Biotechnology, Higher Education Institute of Rab-Rashid, Tabriz, Iran;1. Akdeniz University, Faculty of Medicine, Department of Anatomy, Antalya, Turkey;2. Antalya Bilim University, Faculty of Dentistry, Department of Anatomy, Antalya, Turkey;1. Department of Evolutionary Biology, Ecology and Environmental Sciences, University of Barcelona, Faculty of Biology, Av. Diagonal 643, E-08028 Barcelona, Spain |
| |
| Abstract: | The cellular control of the switch from embryonic to fetal globin formation in man was investigated with studies of globin expression in erythroid cells of 35- to 56-day-old embryos. Analyses of globins synthesized in vivo and in cultures of erythroid progenitors (burst-forming units, BFUe) showed that cells of the yolk sac (primitive) erythropoiesis, in addition to embryonic chains, produced fetal and adult globins and that cells of the definitive (liver) erythropoiesis, in addition to fetal and adult globins, produce embryonic globins. That embryonic, fetal, and adult globins were coexpressed by cells of the same lineage was documented by analysis of globin chains in single BFUe colonies: all 67 yolk sac-origin BFUe colonies and 42 of 43 liver-origin BFUe colonies synthesized epsilon-, gamma-, and beta-chains. These data showed that during the switch from embryonic to adult globin formation, embryonic and definitive globin chains are coexpressed in the primitive, as well as in the definitive, erythroid cells. Such results are compatible with the postulate that the switch from embryonic to fetal globin synthesis represents a time-dependent change in programs of progenitor cells rather than a change in hemopoietic cell lineages. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
