Inhibition of Bacterial Translocation from the Gastrointestinal Tract of Mice Injected with Cyclophosphamide |
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Authors: | Takuya Suzuki Kikuji Itoh Takehiro Hagiwara Hiroyuki Nakayama Kazuhito Honjyo Yoshikazu Hirota Tsutomu Kaneko Hideki Suzuki |
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Institution: | (1) Central Research Institute, Meiji Milk Products, Higashimurayama, Tokyo, 189, Japan , JP;(2) Department of Veterinary Public Health, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan , JP;(3) Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan , JP;(4) Laboratory of Immune Cytology, National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305, Japan , JP |
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Abstract: | Effects of intraperitoneal injection of cyclophosphamide, an immunosuppressant, on the degree of bacterial translocation
and morphological changes of Peyer's patches (PP) in the intestine were investigated with antibiotic-decontaminated SPF mice
and germfree mice monoassociated with Escherichia coli C25. It has been reported that treatment with cyclophosphamide induces bacterial translocation. Cyclophosphamide treatment
in this study, however, significantly decreased E. coli C25 translocation from the gastrointestinal tract to the mesenteric lymph nodes (MLN), although the numbers of lymphoid cells,
especially B cells, in the PP, MLN, and spleen were remarkably reduced. Four injections of cyclophosphamide at a dose of 100
mg/kg inhibited bacterial translocation more than one injection at a dose of 200 mg/kg in SPF mice. Germfree mice, however,
treated with one dose of 200 mg/kg showed the same inhibition of bacterial translocation as those given 100 mg/kg four times.
In cyclophosphamide-treated mice, lymph follicles in the PP were obviously smaller than those in control mice, M-cells were
similar in appearance to absorption epithelial cells except for short microvilli, and immune cells among the M-cells had disappeared.
These data suggested that inhibition of bacterial translocation in mice treated with cyclophosphamide may be the result of
morphological and physiological changes of epithelial cells in the gastrointestinal tract, especially M-cells, as a point
of entry of invading bacteria, independent of the changes in immunological function.
Received: 16 November 1995 / Accepted: 12 December 1995 |
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