Acceleration of Chloroplast Division in Somatic Cell Hybrids between Mesophyll and Cell Culture Protoplasts |
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| Authors: | Kamata, Yasuhiro Kondoh, Katsuhiro Kuroiwa, Tsuneyoshi Nagata, Toshiyuki |
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| Affiliation: | 1Department of Cell Biology, National Institute for Basic Biology Nishigonaka, Myodaiji-cho, Okazaki, 444 Japan
2Department of Biology, Faculty of Science, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113 Japan |
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| Abstract: | Tobacco mesophyll protoplasts were fused with protoplasts fromcultured cells by electric fusion. When the fusion productswere cultured for 2 d, chloroplast division was observed inthe heterokaryocytes under fluorescence microscopy after stainingwith DAPI, while such chloroplast division was not observedwhen mesophyll protoplasts alone were cultured in the same condition. 3Permanent address: Research Institute of House Food IndustryCo. Ltd., Mikuriya Sakaemachi, Higashi-Osaka-shi, Osaka, 577Japan. (Received June 21, 1988; Accepted November 22, 1988) |
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