The biofilm mode of life boosts the anti‐inflammatory properties of Lactobacillus |
| |
Authors: | Aurélie Rieu Nabil Aoudia Gaëtan Jego Johanna Chluba Nadhir Yousfi Romain Briandet Julien Deschamps Benoît Gasquet Vicente Monedero Carmen Garrido Jean Guzzo |
| |
Institution: | 1. UMR A PAM Université de Bourgogne‐AgroSup Dijon – équipe Vin, Aliment, Microbiologie, Stress, , 21000 Dijon, France;2. INSERM, UMR 866, ?Equipe labellisée Ligue contre le Cancer? and Laboratoire d'Excellence LipSTIC, , 21000 Dijon, France;3. INRA, UMR1319 Micalis, , 78352 Jouy‐en‐Josas, France;4. AgroParisTech, UMR Micalis, , 91300 Massy, France;5. CellImaP, Faculty of Medicine and Pharmacy, , 21000 Dijon, France;6. Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), , 46980 Paterna Valencia, Spain;7. University of Burgundy, Faculty of Medicine and Pharmacy, , 21000 Dijon, France;8. Centre anticancéreux George Fran?ois Leclerc, CGFL, , 21000 Dijon, France |
| |
Abstract: | The predominant form of life for microorganisms in their natural habitats is the biofilm mode of growth. The adherence and colonization of probiotic bacteria are considered as essential factors for their immunoregulatory function in the host. Here, we show that Lactobacillus casei ATCC334 adheres to and colonizes the gut of zebrafish larvae. The abundance of pro‐inflammatory cytokines and the recruitment of macrophages were low when inflammation was induced in probiotic‐fed animals, suggesting that these bacteria have anti‐inflammatory properties. We treated human macrophage‐differentiated monocytic THP‐1 cells with supernatants of L. casei ATCC334 grown in either biofilm or planktonic cultures. TNF‐α production was suppressed and the NF‐κB pathway was inhibited only in the presence of supernatants from biofilms. We identified GroEL as the biofilm supernatant compound responsible, at least partially, for this anti‐inflammatory effect. Gradual immunodepletion of GroEL demonstrated that the abundance of GroEL and TNF‐α were inversely correlated. We confirmed that biofilm development in other Lactobacillus species affects the immune response. The biofilms supernatants of these species also contained large amounts of GroEL. Thus, our results demonstrate that the biofilm enhances the immunomodulatory effects of Lactobacillus sp. and that secreted GroEL is involved in this beneficial effect. |
| |
Keywords: | |
|
|