Two-Component Signal Transduction Systems of Desulfovibrio vulgaris: Structural and Phylogenetic Analysis and Deduction of Putative Cognate Pairs |
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Authors: | Weiwen Zhang David E Culley Gang Wu Fred J Brockman |
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Institution: | (1) Microbiology Department, Pacific Northwest National Laboratory, Richland, WA 99352, USA;(2) Department of Biological Sciences, University of Maryland, Baltimore County, Baltimore, MD 21250, USA |
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Abstract: | A large number of two-component signal transduction systems (TCSTS) including 59 putative sensory histidine kinases (HK) and
55 response regulators (RR) were identified from the Desulfovibrio vulgaris genome. In this study, the structural and phylogenetic analyses of all putative TCSTSs in D. vulgaris were performed. The results showed that D. vulgaris contained 21 hybrid-type HKs, implying that multiple-step phosphorelay may be a common signal transduction mechanism in D. vulgaris. Despite the low sequence similarity that restricted the resolution of the phylogenetic analyses, most TCSTS components of
D. vulgaris were found clustered into several subfamilies previously recognized in Escherichia coli and Bacillus subtilis. An attempt was made in this investigation to identify the possible cognate HK-RR pairs not linked on the chromosome in D. vulgaris based on similar expression patterns in response to various environmental and genetic changes. Expression levels of all HK
and RR genes were measured using whole-genome microarrays. Five groups of HK-RR genes not linked on the chromosome were identified
as possible cognate pairs in D. vulgaris. The results provided a preliminary list of possible cognate HK-RR pairs and constitute a basis for further exploration of
interaction and physiological function of TCSTSs in D. vulgaris.
Electronic Supplementary Material Electronic Supplementary material is available for this article at
and accessible for authorised users.
Reviewing Editor: Dr. Gail Simmons] |
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Keywords: | Signal transduction Phylogenetic analysis Microarray Desulfovibrio vulgaris |
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