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Effect of NAD+-dependent formate dehydrogenase on anaerobic respiration of Shewanella oneidensis MR-1 |
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| Authors: | N N Mordkovich T A Voeikova L M Novikova I A Smirnov V K Il’in P E Soldatov A Yu Tyurin-Kuz’min T S Smolenskaya V P Veiko R S Shakulov V G Debabov |
| Institution: | 1. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33/2, Moscow, 119071, Russia 2. State Research Institute of Genetics and Selection of Industrial Microorganisms, Pervyi Dorozhnyi proezd, Moscow, 117545, Russia 3. Institute of Medico-Biological Problems, Russian Academy of Sciences, Khoroshevskoe sh. 76A, Moscow, 123007, Russia |
| Abstract: | An expression plasmid was constructed in order to carry out heterologous expression of the gene of the NAD+-dependent formate dehydrogenase (FDH) from methylotrophic bacterium Moraxella sp. in the cells of Shewanella oneidensis MR-1 under aerobic and anaerobic conditions. In both modes of cell cultivation, recombinant FDH activity was revealed in the cell lysate of the transformants. In the medium with la? tate as a carbon source, the rate of anaerobic respiration determined as the rate of conversion of fumarate (the electron acceptor) to succinate was higher in the transformant with recombinant FDH. Anaerobic cultivation of the FDH-containing transformant of S. oneidensis MR-1 in a microbial fuel cell (MFC) revealed increased current density. |
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