Impaired cell volume regulation in taurine deficient cultured astrocytes |
| |
Authors: | J. Morán T. E. Maar H. Pasantes-Morales |
| |
Affiliation: | (1) Institute de Fisiología Celular, UNAM, Apartado Postal 70-253, 04510 México, D.F. |
| |
Abstract: | Taurine concentration was reduced by 40 and 65%, respectively in rat cerebellar astrocytes grown in a chemically defined medium or in culture medium containing a blocker of taurine transport (GES). Cell volume in these taurine deficient cells was 10%–16% higher than in controls. When challenged by hyposmotic conditions, astrocytes release taurine and this efflux contributes to the volume regulatory decrease observed in these cells. Taurine deficient astrocytes showed a less efficient volume recovery as compared to controls with normal taurine levels. Exposed to 50% hyposmotic medium, astrocytes with normal taurine concentration recovered 60% of their original volume whereas taurine deficient cells recovered only 30–35%. Similarly, in 30% hyposmotic medium, taurine deficient astrocytes recovered only 40% as compared to 75% in controls. No compensatory increases in the efflux of other osmolytes (free amino acids or potassium) were observed during regulatory volume decrease in taurine deficient astrocytes. |
| |
Keywords: | Volume regulatory decrease osmolytes swelling guanidinoethane sulfonate |
本文献已被 SpringerLink 等数据库收录! |
|