Regulated expression of keratan sulphate and peanut agglutinin binding sites during organogenesis in the developing chick |
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Authors: | F J Hemming Raymond Saxod |
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Institution: | (1) Laboratoire de Neurobiologie du Développement, Université Joseph Fourier, CERMO, BP 53, F-38041 Grenoble cedex 9, France e-mail Fiona.Hemming@ujf-grenoble.fr Tel. 33 4 76 51 46 00 ext. 3459; fax 33 4 76 51 49 77, FR |
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Abstract: | Keratan sulphate proteoglycans are potentially important during development and are possible binding molecules for the lectin,
peanut agglutinin, a marker for areas that are inhibitory for axonal growth in early embryos. The present study describes
the spatiotemporal distributions of keratan sulphate epitopes and peanut agglutinin binding sites during organogenesis in
the developing chick from E5 to hatching. The widespread distributions of these molecules did not often overlap but clearly
delimited different carbohydrate compartments demonstrating that peanut agglutinin does not necessarily bind to keratan sulphate
proteoglycans. These markers were mostly extracellular but keratan sulphate, in particular, was found within certain specific
cells in cartilage, gonad, heart and pancreas, at certain ages. The presence of keratan sulphate in putative germ cells during
their migrations and in the gonads may be of particular importance. Their distributions generally evoke modulation of adhesion
allowing cell migrations or morphogenetic movements related to epitheliomesenchymal interactions, but may also suggest an
involvement in axonal guidance in skin, cartilage, gut and possibly heart. Furthermore, in the kidney, peanut agglutinin binding
sites seem to be related to the functional differentiation of the nephrons.
Accepted: 23 February 1998 |
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