Nanoparticle-tethered NAD+ with in situ cofactor regeneration |
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Authors: | Youhui Li Hao Liang Liwei Sun Jianpeng Wu Qipeng Yuan |
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Affiliation: | 1. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China
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Abstract: | A new and simple route for the preparation of immobilized NAD+ on carboxyl-activated silica nanoparticles activated by γ-aminpropyltriethoxysilane and glutaric anhydride was developed. In addition, formate dehydrogenase, keto-reductase and the silica nanoparticle-attached NAD+ were applied to catalyze the coupled reactions for production of l-lactate with the cofactor regenerated within the reaction cycle. As indicated by thermogravimetric analysis and FT-IR, the silica nanoparticles were successfully activated and the loading of carboxyl groups was 0.53 mmol g?1 particle. The amount of immobilized NAD+ on the support was 73 mg g?1 particle. With 0.2 M pyruvate and 3 M formate, 0.16 M l-lactate was produced after the coupled reactions. The immobilized system showed excellent efficiency and stabilities in recycling, and it retained 60 % residual activity even after six reuses. |
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