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Plant regeneration, genetic fidelity, and active ingredient content of encapsulated hairy roots of Picrorhiza kurrooa Royle ex Benth. |
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| Authors: | Janhvi Mishra Rawat Balwant Rawat Shakti Mehrotra |
| Institution: | 1. Molecular Taxonomy Lab, Botany Division, Forest Research Institute (FRI), P.O. New Forest, Dehradun, Uttarakhand, India 3. Society for the Conservation of Nature, Parnkuti Anantpur University Road, Rewa, Madhya Pradesh, India 4. Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India 2. G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India |
| Abstract: | Among five hairy root lines of Picrorhiza kurrooa that were established through Agrobacterium rhizogenes, one (H7) was selected for encapsulation due to high accumulation of picrotin and picrotoxinin (8.3 and 47.6 μg/g DW, respectively). Re-grown encapsulated roots induced adventitious shoots with 73 % frequency on MS medium supplemented with 0.1 μM 6-benzylaminopurine, following 6 months of storage at 25 °C. Regenerated plantlets had 85 % survival after 2 months. Regenerants were of similar morphotype having increased leaf number and branched root system as compared to non-transformed plants. The transformed nature of the plants was confirmed through PCR and Southern blot analysis. Genetic fidelity analysis of transformed plants using RAPD and ISSR showed 5.2 and 3.6 % polymorphism, respectively. Phytochemical analysis also showed that picrotin and picrotoxinin content were similar in hairy root line and its regenerants. |
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