Cloning, overexpression and interaction of recombinant Fur from the cyanobacterium Anabaena PCC 7119 with isiB and its own promoter |
| |
| Authors: | Bes M T Hernández J A Peleato M L Fillat M F |
| |
| Affiliation: | Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Pedro Cerbuna s/n, 50009, Saragossa, Spain. |
| |
| Abstract: | A gene coding for a Fur (ferric uptake regulation) protein from the cyanobacterium Anabaena PCC 7119 has been cloned and overexpressed in Escherichia coli. DNA sequence analysis confirmed the presence of a 151-amino-acid open reading frame that showed homology with the Fur proteins reported for the unicellular cyanobacteria Synechococcus 7942 and Synechocystis PCC 6803. Two putative Fur-binding sites were detected in the promoter regions of the fur gene from Anabaena. Partially purified recombinant Fur binds to the flavodoxin promoter as well as its own promoter. This suggests that the Fur gene is autoregulated in Anabaena. |
| |
| Keywords: | Iron regulation Anabaena Flavodoxin Fur overexpression Autoregulation |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
