大鼠自发性乳腺癌瘤株的建立和病理学研究

[目的]试图建立大鼠自发性乳腺癌产生C型肿瘤病毒的瘤株,探讨大鼠自发性乳腺癌病理学形态特点与C型肿瘤病毒形态学结构。[方法]将大鼠乳腺原发性癌用细胞悬液法和瘤块法交替传代建立瘤株;大鼠乳腺原发性癌和移植性癌分别用10%福尔马林液固定,石蜡包埋切片,HE和Ag染色,光镜观察;分别取大鼠乳腺原发性癌和移植性癌用2.5%戊二醛与1%锇酸双重固定,Epon812包埋,超薄切片,醋酸双氧铀和柠檬酸铅双重染色,透射电镜观察。[结果]大鼠乳腺原发性癌细胞体积较小,直径为10μm,可见小球状结构,乳头状结构,弯曲的条索状与肾小球样结构形成典型的癌性腺管,Ag染色显示癌细胞由网状纤维分隔成不规则巢状,表明大鼠乳腺癌起源于上皮组织,其组织学类型为大鼠乳腺上皮性间变型腺癌。移植性癌细胞呈圆型、立方形、多边形,出现瘤巨细胞与苍白细胞等多种形态,排列成巢状、片状、小梁状、乳头状、小球状、癌性腺管和裂隙状结构,异形性显著。癌细胞形态不规则,细胞核大,核仁增多,胞质中有聚集成束的张力原纤维,罕见分泌颗粒,细胞间存在原始腺腔和桥粒;在瘤细胞间与胞浆内发现了C型肿瘤病毒颗粒,其形态学为球形,直径100nm,外部有囊膜、内膜、中央核心。[结论]首次建立了大鼠自发性乳腺癌产生C型肿瘤病毒的瘤株,其组织学类型为大鼠乳腺上皮性间变型腺癌,病理学形态是多态性的。

Establishment of Rat Spontaneous Mammary Adenocarcinoma Tumor Strain to Generate the Type C Oncovirus and its Pathological Studies

[Objective] The present work is to establish rat spontaneous mammary adenocarcinoma tumor strain to produce the type C oncovirus and investigate the pathological characteristics of rat spontaneous mammary adenocarcinomas caused by type C oncovirus. Methods: The tumor strain of rat mammary adenocarcinomas was established by alternate descent generations of carcinoma struma and cell suspension. For pathological observation, rat mammary primary adenocarcinoma and implant adenocarcinoma were respectively fixed in 10% formaline, embedded in paraffins, routinely stained with HE and Ag. For electron microscope observation, rat mammary primary carcinoma and implant carcinoma were double fixed in 2.5% glutaraldehyde and 1% osmic acid respectively, embedded in Epon 812, ultrathin sectioned, double stained with uranium acetate and lead citrate, and observed under transmission electron microscope (TEM). [Results] The volume of rat mammary primary carcinoma cells is quite small, only 10 (m in diameter. The typical tubular structures are found to be in such shapes as of globe, pappila, tortuousribbon and whorl. The carcinoma cells are separated into irregular nest structure by the reticular fibres after Ag staining. The above-mentioned shows that, as a type of rat mammary epithelial anaplastic adenocarcinomas, rat mammary carcinoma is originated from epithelial tissues. The implant carcinoma cells are round, cubic, and polygonal in shape, and carcinoma gigantic cells, pale cells and the like appeared, which are arranged in such structures as of nest, layer, trabecula, pappila, glomerate, adenocarcinoma tubule, and space, with heteomorphosis notable. The carcinoma cells are irregular in size, with large nuclei and increased nucleolus. Besides, there are bundles of tension protofibril in cytoplasm and seldom seen secretory granules. Among the cells there are primary gland cavities and the desmosome. The type C oncovirus particles are discovered among the carcinoma cells and in their cytoplasm, which are 100nm in diameter, in the shape of globe and with external envelopes endomembranes and central cores. [Conclusion] This is the first time that rat spontaneous mammary adenocarcinomas have been established to generate type C oncovirus tumor strain. It makes up a type of rat mammary epithelial anaplastic adenocarcinomas. And its pathological morphology is of polymorphosm.