Determination of N,N-dimethylarginine in human plasma by high-performance liquid chromatography |
| |
Authors: | Ben-Mei Chen Ling-Wei Xia Rui-Qin Zhao |
| |
Affiliation: | aAnalytical Testing Center, Hunan Medical University, Changsha, Hunan 410078, China;bDepartment of Gynaecology and Obstetrics, The Second Affiliated Hospital, Hunan Medical University, Changsha, Hunan 410005, China |
| |
Abstract: | NG,NG-Dimethylarginine (asymmetric dimethylarginine, ADMA) can be directly separated and measured from deproteinized human plasma using o-phthaldialdehyde-mercaptoethanol (OPA reagent) as a fluorogenic reagent by reversed-phase high-performance liquid chromatography. The mean recovery of ADMA was over 96% and the inter- and intra-assay coefficients of variation of amounts were lower than 3.80% and those of retention time were below 0.37% for five runs. The detection limit of the assay is 1 pmol when the signal-to-noise is 3:1. It was observed that the concentration of ADMA was significantly elevated in plasma of patients with pregnancy induced hypertension (PIH) in contrast to healthy pregnant women. |
| |
Keywords: | Dimethylarginine |
本文献已被 ScienceDirect 等数据库收录! |
|