Stabilization of the immobilized linkers and DNA probes for DNA microarray fabrication by end-capping of the remaining unreacted silanol on the glass |
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| Authors: | Nagendra Kumar Kamisetty Seung Pil Pack Mitsuru Nonogawa Kazunari Yamada Yasuko Yoshida Tsutomu Kodaki Keisuke Makino |
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| Institution: | aInstitute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan;bCREST, JST (Japan Science and Technology Agency), Kyoto University, Gokasho, Uji 611-0011, Japan;cDepartment of Biotechnology and Bioinformatics, Korea University, Jochiwon, Chungnam 339-700, South Korea;dNGK Insulators Ltd., Future Technology Management Center, Mizuho, Nagoya 467-8530, Japan |
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| Abstract: | High stability of the oligonucleotides immobilized on the glass is essential for the reliable DNA microarray analysis. In the present study, effect of end-capping of the unreacted silanol, remaining after the surface amine-functionalization, was explored: (1) Cy3-NHS (N-hydorxysuccinincimide) dye was spotted on the surface and change in the fluorescent intensity was measured. (2) DNA probes were immobilized by the reactivity of oxanine linked at the 5’-end, the complementary oligonucleotides with Cy5-fluorescence at the 5’-end was hybridized, and the time-dependence of the fluorescence intensity was observed. Both the systems showed improved stability of the immobilized molecules, indicative of the stabilization by end-capping. |
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| Keywords: | End-capping Amine-functionalized surface DNA microarray DNA immobilization Surface modification |
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