Genetic diversity of Iranian <Emphasis Type="Italic">Aegilops tauschii</Emphasis> Coss. using microsatellite molecular markers and morphological traits |
| |
Authors: | Zahra Tahernezhad Mohammad Javad Zamani Mahmood Solouki Mehdi Zahravi Abbas Ali Imamjomeh Mohammad Jafaraghaei Mohammad Reza Bihamta |
| |
Institution: | (1) Molecular Marker Lab, Seed and Plant Certification and Registration Institute, Karaj, Iran;(2) Seed and Plant Improvement Institute, Karaj, Iran;(3) Department of Agronomy & Plant Breeding, University of Zabol, Zabol, Iran;(4) National Plant Gene Bank of Iran, Karaj, Iran;(5) Department of Agronomy and Plant breeding, Agriculture College, Tehran University, Karaj, Iran |
| |
Abstract: | Aegilops tauschii Coss. is a diploid (2n = 2x = 14,DD) goat grass species which has contributed the D genome in common wheat. Genetic variations
in 28 accessions of Aegilops tauschii belonged to different provinces of Iran, were evaluated using 16 morphological traits and 19 SSR markers. In number of spikelet
per spike and plant height, there was a high variation in ssp. tauschii and ssp. strangulata respectively and for days to mature a low variation in both subspecies was found. Discriminant function analysis showed that
67.9% of original grouped cases correctly classified. Factor analysis indicated that three factor explain 66.49% of total
variation. The three clusters revealed by the cluster analysis were not consistent with their geographical distributions.
We determined 208 alleles using 19 microsatellites. Average of alleles for every locus was 10.94. The total average of PIC
was 0.267. 2261 bands produced for total of genotypes and Chinese Spring had the highest bands (95 alleles). The range of
similarity coefficients was between 0.23 and 0.73. Genotypes were clustered using UPGMA method. The accessions did not match
according to morphological cluster and geographical regions. 51.2% of total variations were related to 9 principle components. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|