Showing their true colors: a practical approach to volume rendering from serial sections |
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Authors: | Stephan Handschuh Thomas Schwaha Brian D Metscher |
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Institution: | 1.Department of Theoretical Biology (Gerd Müller, Head),University of Vienna,Vienna,Austria |
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Abstract: | Background In comparison to more modern imaging methods, conventional light microscopy still offers a range of substantial advantages
with regard to contrast options, accessible specimen size, and resolution. Currently, tomographic image data in particular
is most commonly visualized in three dimensions using volume rendering. To date, this method has only very rarely been applied
to image stacks taken from serial sections, whereas surface rendering is still the most prevalent method for presenting such
data sets three-dimensionally. The aim of this study was to develop standard protocols for volume rendering of image stacks
of serial sections, while retaining the benefits of light microscopy such as resolution and color information. |
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