A genomics-informed,SNP association study reveals FBLN1 and FABP4 as contributing to resistance to fleece rot in Australian Merino sheep |
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Authors: | Wendy JM Smith Yutao Li Aaron Ingham Eliza Collis Sean M McWilliam Tom J Dixon Belinda J Norris Suzanne I Mortimer Robert J Moore Antonio Reverter |
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Institution: | (1) CSIRO Livestock Industries, 306 Carmody Rd, St Lucia, QLD 4067, Australia;(2) Industry and Investment NSW, Agricultural Research Centre, Trangie, NSW, 2823, Australia;(3) SheepGENOMICS, Wool Sub-Program, 306 Carmody Rd, St Lucia, QLD 4067, Australia;(4) Australian Animal Health Laboratory, CSIRO Livestock Industries, Private Bag 24, Geelong, Vic. 3220, Australia |
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Abstract: | Background Fleece rot (FR) and body-strike of Merino sheep by the sheep blowfly Lucilia cuprina are major problems for the Australian wool industry, causing significant losses as a result of increased management costs
coupled with reduced wool productivity and quality. In addition to direct effects on fleece quality, fleece rot is a major
predisposing factor to blowfly strike on the body of sheep. In order to investigate the genetic drivers of resistance to fleece
rot, we constructed a combined ovine-bovine cDNA microarray of almost 12,000 probes including 6,125 skin expressed sequence
tags and 5,760 anonymous clones obtained from skin subtracted libraries derived from fleece rot resistant and susceptible
animals. This microarray platform was used to profile the gene expression changes between skin samples of six resistant and
six susceptible animals taken immediately before, during and after FR induction. Mixed-model equations were employed to normalize
the data and 155 genes were found to be differentially expressed (DE). Ten DE genes were selected for validation using real-time
PCR on independent skin samples. The genomic regions of a further 5 DE genes were surveyed to identify single nucleotide polymorphisms
(SNP) that were genotyped across three populations for their associations with fleece rot resistance. |
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