Association of specific tyrosine phosphorylation with stages of B-cell differentiation in human lymphoid leukemias |
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| Authors: | H Kuratsune K Owada T Machii Y Nishimori E Ueda Y Tokumine S Tagawa N Taniguchi H Fujio T Kitani |
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| Affiliation: | 1. Department of Clinical Research (Division of Internal Medicine), The Research Institute for Microbial Diseases, Osaka University, 3-1, Yamadaoka, Suita-City, Osaka, Japan;2. Department of Tumor Virology, The Research Institute for Microbial Diseases, Osaka University, 3-1, Yamadaoka, Suita-City, Osaka, Japan;3. Department of Immunochemistry, The Research Institute for Microbial Diseases, Osaka University, 3-1, Yamadaoka, Suita-City, Osaka, Japan;1. Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan;2. Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei, Taiwan;3. Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung, Taiwan;4. College of Engineering, Chang Gung University, Taoyuan, Taiwan;1. General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China;2. Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China |
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| Abstract: | In vitro protein phosphorylation in various types of human fresh lymphoid leukemic cells (C-ALL, B-CLL, HCL and PCL: B-cell lineage and T-ALL, ATL and T-CLL: T-cell lineage) were studied. In cases of B-CLL and HCL, tyrosine protein kinase (TPK) activity was at least 5-fold higher than that in other cases of B- and T-cell lineages. B-cell leukemic cells at various differentiation stages had different endogenous substrates in tyrosine phosphorylation as well as distinct TPK activity. The P-tyr-containing proteins of 68K, 59K and 56K were detected commonly in all the cases of B-cell lineage. The phosphorylated protein of 32K was present only in cases of PCL. On the other hand, in T-ALL and ATL, the major substrate in tyrosine phosphorylation was 58K. These results suggest that the characterization of in vitro tyrosine phosphorylation provides a new means not only to distinguish T- and B-lymphoid leukemia, but also to differentiate stages of lymphoid development. |
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