Polyamines content and physiological and biochemical responses to ladder concentration of nickel stress in Hydrocharis dubia (Bl.) Backer leaves |
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Authors: | Juan Zhao Guoxin Shi Qiuhong Yuan |
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Institution: | (1) College of Life Science, Nanjing Normal University, Nanjing, Jiangsu, 210046, China |
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Abstract: | Influence of ladder concentration of nickel (Ni) on the leaves of Hydrocharis dubia were studied after 3 days treatment. The accumulation of Ni, the content of polyamines, proline, malondialdehyde (MDA) and
soluble protein, as well as the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in the leaves
were investigated. The result indicated that the toxicity of Ni manifested in respective aspect of physiological and biochemical
characters. Significant increase of Ni concentration in the leaf tissue was observed, which was concentration dependent. Visible
symptoms of Ni toxicity: chlorosis and necrosis occurred following the 3rd day. Meantime, treatment with Ni resulted in the
increase in the generation rate of O2•− in the leaves. SOD and CAT activities decreased significantly in response to Ni treatment, it was possibly the reason of
accumulation of O2•−. However, a several-fold decrease in POD activities was found. Our results indicated that because of prolonged increases
in O2•− level, oxidative damage, measured as the level of lipid peroxidation, occured in the leaves of Ni treated fronds. The changes
of the content of polyamines (PAs) were also investigated in the leaves of Hydrocharis dubia. Ni treatment significantly increased the putrescine (Put) level and lowered spermidine (Spd) and spermine (Spm) levels,
thereby significantly reducing the ratio of free (Spd + Spm)/Put in leaves, which has been considered as the signal under
stress. Although the trend that PS-conjugated PAs and PIS-bound PAs changed the same as free PAs, they changed in more less
extent. |
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Keywords: | Nickel Polyamine Physiology Stress Hydrocharis dubia |
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