Restoration of membrane potential in mitochondria deenergized with carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) |
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| Authors: | A. Toninello N. Siliprandi |
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| Affiliation: | 1. Institute of Biological Chemistry, University of Padua, 35100 PadovaItaly;2. CNR Unit for the Study of Mitochondrial Physiology, 35100 PadovaItaly |
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| Abstract: | The membrane potential (Δψ) of rat liver mitochondria dropped upon addition of carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) but was gradually and fully restored to the original value by the subsequent addition of dithioerythritol. Concomitantly, Ca2+ released from mitochondria was reaccumulated and the oxidative phosphorylation process completely recoupled. Neither of these effects has been observed with dinitro-o-cresol or 2,4-dinitrophenol, uncouplers which, unlike FCCP, do not react with thiols. Δψ abolished by FCCP was also restored, though incompletely, by albumin; a prompt and complete restoration was however achieved upon subsequent addition of dithioerythritol. Dithioerythritol also completely and rapidly restored the Δψ decreased by addition of diazene dicarboxylic acid bisdimethylamide (diamide). |
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| Keywords: | Membrane potential Deenergization Uncoupler Thiol reagent CCCP diamide diazene dicarboxylic acid bisdimethylamide FCCP Hepes 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid tetraphenylphosphonium |
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