On the quantitation of bacteriochlorophyll in chromatophore suspensions from purple bacteria |
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| Authors: | Hartmut Neufang Horst Müller Karl Knobloch |
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| Affiliation: | Institut für Botanik and Pharmazeutische Biologie der Universität Erlangen-Nürnberg, Schlossgarten 4, D-8520 Erlangen F.R.G. |
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| Abstract: | A direct photometric quantitation of bacteriochlorophyll (BChl) at 375 nm in aqueous chromatophore suspensions from various purple bacteria is described. The assay is rapid and reproducible. It is utilized easily for processing large numbers of samples and is as sensitive as extraction methods usually applied today. Drawbacks of extraction methods, particularly not quantitative extractions, photo- and autooxidation are avoided. There is good linearity up to 20 μg BChl/ml suspension, and no interference by buffers is observed. |
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| Keywords: | Bacteriochlorophyll Chromatophore Bacterial photosynthesis (Purple bacteria) BChl bacteriochlorophyll |
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