Double-phase culture system for large scale production of pineapple |
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| Authors: | Jonny E Scherwinski-Pereira Elequisandra da C Araruna Lima Tatiane L da Silva Antonio G Gomes Mesquita Simone de A Maciel Frederico H da Silva Costa |
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| Institution: | 1.Embrapa Genetic Resources and Biotechnology,Brasília,Brazil;2.Federal University of Uberlandia,Uberlandia,Brazil;3.Luiz de Queiroz College of Agriculture,Piracicaba,Brazil;4.Federal University of Acre,Rio Branco,Brazil;5.University of Western S?o Paulo,Presidente Prudente,Brazil |
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| Abstract: | We have developed an efficient and simplified method for shoot proliferation of the pineapple (Ananas comosus L.), using a Double-Phase Culture System (DPS) (semi-solid medium with a layer of liquid medium on the top). Under these
conditions, up to 430 microshoots were produced from 5 initial shoots within 5 months, and without shoot manipulation. Thus,
>50% shoots proliferated as compared to that on agar-solidified medium. Shoots taken from DPS were rooted within 4 weeks at
a frequency of up to 100%, in full-strength or half-strength Murashige and Skoog medium containing 0.5–2.5 μM indole-3-butyric
acid. The plantlets were then transferred into soil, and they survived acclimatization with 100% success. Thus, DPS could
be useful for large-scale micropropagation of a range of pineapple cultivars, with high production of plantlets at the end
of process, and reduced labor costs during the subcultures. |
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