The expression changes of Numblike in rat brain cortex after traumatic brain injury |
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| Authors: | Shengyang Jiang Xiaohong Wu Yaohua Yan Jian Xu Bai Shao Xun Zhuang Yingying Han Xiaosong Gu |
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| Institution: | (1) Department of Anatomy, School of Basic Medical and Biological Sciences, Medical College of Soochow University, Soochow University, Suzhou, 215021, People’s Republic of China;(2) School of Public Health, Nantong University, Nantong, 226001, People’s Republic of China;(3) Department of Neurosurgery, Affiliated Hospital of Nantong University, Medical College, Nantong University, Nantong, 226001, People’s Republic of China;(4) Key Laboratory of Neuroscience, Nantong University, Nantong, 226001, People’s Republic of China; |
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| Abstract: | Numblike (Numbl) plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. And Numbl
is specifically expressed in the brain. However, its expression and function in the central nervous system lesion are still
unclear. In this study, we performed a traumatic brain injury (TBI) model in adult rats and investigated the dynamic changes
of Numbl expression in the brain cortex. Western blot and immunohistochemistry analysis revealed that Numbl was present in
normal brain. It gradually decreased, reached the lowest point at day 3 after TBI, and then increased during the following
days. Double immunofluorescence staining showed that Numbl immunoreactivity was found in neurons, but not astrocytes and microglia.
Moreover, the 3rd day post injury was the apoptotic peak implied by the alteration of caspase-3. All these results suggested
that Numbl may be involved in the pathophysiology of TBI and further research is needed to have a good understanding of its
function and mechanism. |
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