Production of lyophilized culture ofLactobacillus acidophilus with preserving cell viability |
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Authors: | Moo Heon Kang Vibhor Saraswat Jeewon Lee Young-Hoon Park |
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Affiliation: | (1) Bioprocess Eng. Lab., Hanhyo Institutes of Technology, Yusong, 305-309 Taejon, Korea;(2) Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, P. O. Box 115, 305-600 Taejon, Korea |
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Abstract: | Optimal lyophilization process was developed for manufacturing the dried product ofLactobacillus acidophilus with high cell viability. Three major factors, freezing rate, specific surface area of samples, and stabilizer type and their synergy were shown to play a crucial role in the development of an effective lyophilization process. Finally we found an optimal combination among three process parameters mentioned above: an exceptionally high cell survival percentage of 90% was achieved using the 8.28 cm−1 specific surface area of samples, slow freezing rate, and a stabilizer composition of 4% skim milk+1% glycerol +0.1% calcium chloride. |
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Keywords: | Lactobacillus acidophilus lyophilization survival percentage |
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