Induction of rat alpha 2-macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell-derived factor |
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Authors: | J Bauer M Birmelin G H Northoff W Northemann T A Tran-Thi H Ueberberg K Decker P C Heinrich |
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Affiliation: | 1. Biochemisches Institut, Universität Freiburg, Hermann-Herder-Str. 7, D-7800 FreiburgGermany;2. Blutspendezentrale des DRK, Oberer Eselsberg, D-7900 UlmGermany;3. Dr. Karl Thomae GmbH. Birkendorf er-Str. 65, D-7950 Biberach, FRG |
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Abstract: | Turpentine injection into rats elicits enhanced secretion of acute phase proteins including alpha 2-macroglobulin (alpha 2M). Hypophysectomized rats, however, do not respond in this way unless dexamethasone is given together with turpentine. On the other hand, dexamethasone injection alone did not result in an induction of alpha 2M synthesis. When a medium of Kupffer cell cultures was added to hepatocytes, a dose-dependent stimulation of alpha 2M synthesis of up to 4-fold after 10-12 h was observed. However, the presence of low concentrations (10(-9)M) of dexamethasone was essential for the stimulatory effect. We conclude that the acute phase induction of alpha 2M in hepatocytes requires the synergistic action of glucocorticoids and a non-dialysable factor secreted by Kupffer cells. |
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Keywords: | Acute-phase protein Glucocorticoid Hepatocyte-stimulating factor Kupffer cell AT III, antithrombin III TF, transferrin LPS, lipopolysaccharide SDS-PAGE, SDS-polyacrylamide gel electrophoresis |
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