Mechanism of electron transport in the cholesterol-hydroxylating system of adrenal cortex mitochondria: a triple complex of adrenodoxin reductase, adrenodoxin and cytochrome P-450

A cross-linked ternary adrenodoxin reductase-adrenodoxin-cytochrome P-450scc complex with an apparent molecular mass of 114 kD was obtained, using N-succinimidyl-6-(4'-azido-2'-nitrophenylamino)-hexanoate. The composition of the cross-linked complex was determined by immunoblotting and radioactivity measurements, using N-ethyl 2.3-14C]maleimide-premodified adrenodoxin. The data obtained suggest that the ternary complex may exist in solution.