Virus passage through track-etch membranes modified by salinity and a nonionic surfactant. |
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| Authors: | C D Lytle L B Routson N B Jain M R Myers B L Green |
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| Institution: | Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 20857, USA. cdl@cdrh.fda.gov |
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| Abstract: | Why do viruses sometimes not pass through larger pores in track-etch filters? Increasing the salinity (0.8 to 160 mM Na+) decreased phiX174 and PRD1 passage through track-etch polycarbonate membranes (sodium dodecyl sulfate coated but not polyvinylpyrrolidone coated) and PRD1 passage through polyester membranes. Undiminished passage when 0.1% Tween 80 was added implied that nonionic virus adsorption occurred and indicated that high levels of salinity decreased virus passage by decreasing electrostatic repulsion that prevented adsorption. |
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