In vitro establishment of a cycloclonal chain from nodal segments and apical buds of adult hazel (Corylus avellana L.) |
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Authors: | C Díaz-Sala M Rey R Rodríguez |
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Institution: | (1) Lab. Fisiología Vegetal, Departamento B.O.S., Facultad de Biología, Universidad de Oviedo, 33005 Oviedo, Spain |
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Abstract: | Apical buds (0.5 cm) and nodal shoot segments (1.5 cm) excised from: A) field-grown branches, B) newly developed shoots from the forced outgrowth of axillary buds on A branches, C) newly developed shoots from the forced outgrowth of axillary buds on A branches submitted to cold storage were used as primary explants. Results indicate that three months cold storage greatly increases morphogenic capacity and reduces contamination and oxidation of tissues. Consequently, a multiplying chain could be easily established by culturing the tissues on a modified Murashige & Skoog (1962) medium plus 6-benzyl-aminopurine 5 mg l-1, indole-3-acetic acid 0.01 mg l-1 and gibberellic acid 0.1 mg l-1. During the initiation and proliferation phases, both the proliferation and the elongation rate were significantly increased when a double-phase culture system (Viseur 1987) was used, giving rise to a higher microplant production than the one obtained using previously described methods. Plant regeneration was achieved by immersing the single microshoot's basal end in an IBA (0.1–1 mg ml-1) solution for 10 s followed by a 20-day culture on a 1/2 MS2 medium.Abbreviations BAP
6-benzylaminopurine
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- 2ip
2-isopentenyladenine
- MS1 and MS2
modified Murashige & Skoog media
- NAA
2-maphtaleneacetic acid |
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Keywords: | double-phase culture system hazel in vitro tissue culture micropropagation woody species |
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