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Effect of phosphorus supply on periplasmic protein profiles in Rhizobia |
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| Authors: | Smart J B Dilworth M J Robson A D |
| Institution: | (1) Nitrogen Fixation Research Group, School of Environmental and Life Sciences, Murdoch University, 6150 Murdoch, Western Australia;(2) Soil Science and Plant Nutrition Group, School of Agriculture, University of Western Australia, 6009 Nedlands, Western Australia, Australia;(3) New Zealand Dairy Research Institute, Private Bag, Palmerston North, New Zealand |
| Abstract: | Lysozyme/EDTA treatment of four fast-growing rhizobia released repeatable protein profiles after polyacrylamide slab gel electrophoresis. Similar treatment of slow-growing rhizobia failed to release such periplasmic proteins.For the four-fast-growing rhizobia, both P-repressible and P-inducible protein bands occurred. The only P-repressible protein identified was alkaline phosphatase, which showed strain differences in both electrophoretic mobility and activation by Mg2+.The derepression of the P-repressible periplasmic proteins in cowpea Rhizobium NGR234 correlated with derepression of both phosphate and glycerol 1-phosphate uptake.Abbreviation HEPES
N-2-hydroxyethylpiperazine-N -2-ethanesulphonic acid |
| Keywords: | Alkaline phosphatase Continuous culture Periplasmic proteins Phosphate uptake Phosphorus nutrition Rhizobium |
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