A simple assay for the quantitative evaluation of Phospholipase D activity |
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| Authors: | Rosanna Cardillo Paola D'Arrigo Lorenzo De Ferra Valentine Piergianni Domenico Scarcelli Stefano Servi |
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| Affiliation: | (1) Dipartimento di Chimica, Politecnico di Milano, CNR, Centre per lo Studio delle Sostanze Organiche Naturali, Via Mancinelli 7, 20131 Milano, Italy;(2) Italfarmaco Sud S.p.A., Via Vadisi 5, 03010 Patrica, (Fr), Italy |
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| Abstract: | Summary We describe the preparation of phosphatidyl p-nitrophenol (PpNP) and its use as a substrate in an assay for the quantitative evaluation of Phospholipase D. The solution of the substrate in Tris HCl buffer, pH 8, and Triton X is mixed directly with an aliquot of fermentation broth and the absorbance of the solution read at 405 nm. The method is rapid, sensitive and inexpensive. |
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