Plant regeneration from callus of Puccinellia distans (L.) Parl |
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Authors: | D. Q. Binh L. E. Heszky G. Gyulai E. Kiss A. Csillag |
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Affiliation: | (1) Department of Genetics and Plant Breeding, University of Agriculture, H-2103 Gödöll, Hungary;(2) Department of Central Laboratory, University of Horticulture and Food Industry, H-1118 Budapest, Hungary |
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Abstract: | Callus was induced from seeds of Puccinellia distans (L.) Parl. on MS medium supplemented with 2 mgl-1 2,4-dichlorophenoxyacetic acid and 0.5 mgl-1 kinetin. Morphogenesis initiation was achieved during subculture on medium containing 0.1 mgl-1 2,4-D. From the point of morphogenetic capacity, 3 types of callus were selected. High frequency of plant regeneration was obtained by selection of embryogenic type of callus, and culture on N6 medium and N6 medium supplemented with kinetin (5–10 mgl-1), or kinetin (2 mgl-1) and IAA (0.5 mgl-1). A high ratio of albinos among regenerants was observed. |
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Keywords: | callus-type plant regeneration Puccinellia distans |
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