Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of a Burkholderia cepacia strain collection |
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Authors: | Olfa Kilani-Feki Imen Zouari Gérald Culioli Annick Ortalo-Magné Nabil Zouari Yves Blache Samir Jaoua |
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Institution: | (1) Equipe ? Biopesticides ? L.P.A.P, Centre de Biotechnologie de Sfax, Universit? de Sfax, P.O. Box 1177, Sfax, 3018, Tunisie;(2) Biological and Environmental Sciences Department, College of Arts and Sciences, Qatar University, P.O. Box: 2713, Doha, Qatar;(3) Laboratoire MAPIEM (EA 4323), Universit? du Sud Toulon-Var, 83957 La Garde Cedex, France; |
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Abstract: | Twenty epiphytic and rhizospheric bacterial strains harbouring strong antifungal activities were isolated from the Tunisian
environment. This group of bacteria was identified as Burkholderia cepacia genomovar I using 16S rDNA and recA fragment gene sequence analyses for two selected strains and RFLP technique for the eighteen other ones. This identification
did not show variability between isolates despite the significant differences in the antifungal activities of their culture
supernatant and the organic crude extract against Aspergillus niger and other phytopathogenic fungi. Chromatographic and mass spectrometric analyses of these extracts allowed us to confirm
the difference between strains of the group. Their metabolic production showed differences in term of contents and quantities
of secreted molecules, particularly those which were identified to be involved in the antifungal activities. Two metabolites,
named Bc-255 and Bc-257 secreted by the entire group at different amounts, have been purified and tested separately against
A. niger. Bc-255 showed an activity twice as high as those shown by Bc-257. The structural characterization of these two compounds
by mass spectrometry and nuclear magnetic resonance spectroscopy allowed their identification as two analogous 2-alkylquinolones
with only one difference at the alkyl chain. |
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