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灵芝转化苦参水提物的3个新生成分体外抗乙型肝炎病毒作用
引用本文:李雁群 章克昌. 灵芝转化苦参水提物的3个新生成分体外抗乙型肝炎病毒作用[J]. 微生物学报, 2005, 45(4): 643-646
作者姓名:李雁群 章克昌
作者单位:1. 华南师范大学生命科学学院,广州,510631
2. 江南大学生物工程学院,无锡,214036
摘    要:先在基础培养基中添加苦参水煎汁,然后培养灵芝,得到灵芝培养液,再按乙醇氯仿-5%碳酸氢钠溶液-氯仿的顺序提取培养液中的有机酸成分,用制备高效液相色谱分离,得到6个新组分,用这些组分分别作用于转染了乙型肝炎病毒DNA的2.2.15细胞,用固相放射免疫法测定细胞培养上清液中的乙肝病毒表面抗原(HBsAg)和e抗原(HBeAg)的含量,用四甲基偶氮唑盐(MTT)法测定细胞的存活率。结果表明,其中的3个组分对2.2.15细胞分泌HBsAg和HBeAg有抑制作用,提示可能具有抗乙肝病毒的作用。

关 键 词:灵芝  苦参  乙肝  液体培养  抗病毒
文章编号:0001-6209(2005)04-0643-04
修稿时间:2004-12-23

In vitro inhibitory efects on HBsAg and HBeAg secretion of 3 new components produced by Ganoderma lucidum in the medium contained Radix sophorae flavescentis extract
LI Yan-qun,ZHANG Ke-chang. In vitro inhibitory efects on HBsAg and HBeAg secretion of 3 new components produced by Ganoderma lucidum in the medium contained Radix sophorae flavescentis extract[J]. Acta microbiologica Sinica, 2005, 45(4): 643-646
Authors:LI Yan-qun  ZHANG Ke-chang
Affiliation:College of Life Science, South China Normal University, Guangzhou 510631, China. liyq2004@126.com
Abstract:In order to enhance the medical effects of Ganoderma lucidum submergedly cultured broth, the aqueous extract of Radix sophorae flavescentis, a traditional Chinese medicine, was added into the cultivation medium of G. lucidum. The organic acids were extracted with ethanol, chloroform, 5% NaHCO-3 and chloroform in turn from the cultured broth of Ganoderma lucidum which cultivation medium contained Radix sophorae flavescentis extract. Six new components were separated from the organic acids with preparative HPLC. Their inhibitory effects on HBsAg and HBeAg secretion of HBV DNA transferred HepG2 cell (2.2.15 cell) were investigated. The results indicate that 3 components of the six have significant inhibitory effects on the antigen secretion.
Keywords:Ganoderma lucidum   Radix sophorae flavescentis   Hepatitis B Virus   Submerged culture   Anti-virus  
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