Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines |
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Authors: | B Malissen M P Price J M Goverman M McMillan J White J Kappler P Marrack A Pierres M Pierres L Hood |
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Institution: | 1. Division of Biology California Institute of Technology Pasadena, California 91125 USA;2. Department of Microbiology University of Southern California Medical School Los Angeles, California 90033 USA;3. Department of Medicine National Jewish Hospital and Research Center Denver, Colorado 80206 USA;4. Centre d''Immunologie de Marseille-Luminy Marseille Cedex 9, France |
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Abstract: | We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional gel electrophoresis. Furthermore, the I-Ak molecules on L cells can act as targets for the allogenic T-cell killing of the transformed L cells. The I-Ak molecules in both mouse fibroblasts and hamster B cells can present certain antigens to T-cell helper hybridomas. Thus only class II molecules are required to convert the nonantigen-presenting cell. Accordingly, it will be possible to dissect the structure-function relationships existing between Ia molecules, foreign antigen, and T-cell receptor molecules by in vitro site-directed mutagenesis and gene transfer. |
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