用斑点杂交法比较小麦全蚀菌病毒与其它真菌病毒的同源性

HOMOLOGY BETWEEN GAEUMANNOMYCES GRAMINIS VIRUS AND OTHERMYCOVIRUSES BY DOT BLOT HYBRIDIZATION

Using labelled dsRNA of Gaenmannomyces 9raminis virus Taian ( GgV-Taian ) as probe, dot blot analysis was made between GgV-Taian and viruses from five species of fungus or seven isolates of GgV. The dsRNA of GgV-Taian hybridized with viral dsRNA of distantly related species of As-per3illus oryzae, A. niger, Pleurotus sapid us and dsRNA of GgV collected from Taian, Yantai, Jiangsu, Zhejiang; but not with dsRNA of GgV-Hubei, GgV-Qinghai and Volvariella volvacea virus.Comparison of the results of the dot blot hybridization with those of the serological test among these viruses showed that the antiserum to GgV-Taian reacted with viruses of A, oryzae and P. sapidus, but not with A, niger virus. However, there was strong cross hybridization between A. ni-9er virus and GgV-Taian. The results give new evidences of the homology of viruses in taxonomi cally distantly related fungal species.