| 一种安全有效的卵母细胞封闭式玻璃化冷冻载体 |
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| 引用本文： | 冯贵雪,方伟芬,张波,舒金辉,周红,周莉,甘贤优,刘茵. 一种安全有效的卵母细胞封闭式玻璃化冷冻载体[J]. 中国实验动物学杂志, 2009, 0(1): 46-49 |
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| 作者姓名： | 冯贵雪 方伟芬 张波 舒金辉 周红 周莉 甘贤优 刘茵 |
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| 作者单位： | 广西壮族自治区妇幼保健院生殖中心,南宁530003 |
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| 基金项目： | 广西自然科学基金资助项目（桂科自0542058）和广两卫生厅科研项目（Z2004024,Z2007013,Z2007012）. |
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| 摘 要： | 目的探讨封闭式玻璃化冷冻载体冻存小鼠卵母细胞的可行性。方法以小鼠MII期卵母细胞为模型,以开放式玻璃微细管法（GMP）为对照组,比较两种玻璃化冷冻载体对小鼠卵母细胞冷冻后的存活率、受精率、卵裂率及囊胚率的影响。结果卵母细胞经冻融后,封闭式冷冻载体组和GMP组的存活率、受精率、卵裂率和囊胚率均没有明显差异（92.80%vs93.11%,49.80%vs51.67%,36.73%vs35.83%,12.65%vs14.17%%;P〉0.05）。结论封闭式冷冻载体能安全、有效的冷冻保存小鼠卵母细胞。
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| 关 键 词： | 小鼠 卵母细胞 玻璃化冷冻 冷冻载体 |
A Safe and Effective Close-carrier of Vitrificaton of Mature Mouse Oocytes |
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| FENG Gui-xue,FANG Wei-fen,ZHANG Bo,SHU Jin-hui ZHOU Hong,ZHOU El,GAN Xian-you,LIU Yin. A Safe and Effective Close-carrier of Vitrificaton of Mature Mouse Oocytes[J]. Chinese Journal of Laboratory Animal Science, 2009, 0(1): 46-49 |
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| Authors: | FENG Gui-xue FANG Wei-fen ZHANG Bo SHU Jin-hui ZHOU Hong ZHOU El GAN Xian-you LIU Yin |
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| Affiliation: | ( Reproductive Medicine Center, Maternal and Child Health Hospital of Guangxi, Nanning 530003, China) |
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| Abstract: | Objective To explore the feasibility of vitrification of mouse maturation oocytes, using a close-carrier. Methods The survival rate, fertilization rate, cleavage rate and blastoeyst rate of vitrification using a close-carrier were compared with that of using glass micropipette （GMP） . Results There were no significantly differences between the close-carrier and GMP in the survival, fertilization, cleavage and blastocyst rates respectively （92.80 % vs 93.11 % , 49.80% vs 51.67% , 36.73 % vs 35.83% ,12.65 % vs 14.17% %; P 〉 0.05）. Conclusion It is effective and safe for cryopreservation of mouse maturation oocyte using the close-carrier. |
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| Keywords: | Oocytes Vitrification Mouse Cryopreservation carrier Glass micropipette （GMP） |
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