双表达小干扰RNA抑制小鼠体内HBV感染

目的运用水压法复制HBV小鼠急性模型,用于研究双表达siRNA在小鼠体内对HBV复制的抑制作用。方法使用HBV质粒DNA对BALB/c小鼠进行尾静脉大剂量快速推注,获得急性HBV体内表达模型。同时使用相同方法对推注后的小鼠进行RNAi干预。对不同处理组动物的HBsAg、HBeAg、HBcAg和HBV DNA进行ELISA、免疫组织化学和实时定量RT-PCR检测。结果水压法复制HBV小鼠急性模型与HBV DNA感染剂量无明显相关。与单表达siRNA相比较,双表达siRNA对HBV抗原表达抑制较为明显。结论双表达siRNA不失为一种体内抑制HBV感染的一种策略。

Inhibition of HBV Infection in Mouse Model by Dual Small Interfering RNA Treatment

Objective To explore the therapeutic potential of RNAi on mutation in HBV genome resulting in resistance when small interfering RNA （siRNA） is luther developed as an anti-viral drug, we express simultaneously two different 21-bp hairpin siRNA duplexes that specifically attack the HBs and HBx genes of HBV, respectively, in vivo. Methods We copied the acute HBV mice model in the study, using hydrodynamics with the mouse tail. The same technique was used to treat with interfering RNA in vivo. The antigens and DNA of HBV were determined with ELISA, Immunohistochemical staining and real-time RT-PCR. Results Dual siRNA could inhibit simultaneously the level of viral DNA and viral antigens detected in the livers and sera of the treated mice relative to control animals. Conclusion This dual siRNA system provides a more powerful tool to inhibit HBV replication in vivo.